Description
Surveys the biology, diagnosis, and treatment of ovarian cancer, and provides step-by-step instructions for molecular biology techniques of understanding cancer. Techniques are designed to determine tumor genetics, expression, and protein function, and to elucidate the genetic mechanisms by which gene and immunotherapies may be perfected. Chapters are in sections on the epidemiology of ovarian cancer, markers of tumor burden, model systems, cytogenetics, molecular genetic imbalances in ovarian tumors, and mRNA analysis. Other subjects are protein expression, signal transduction, apoptosis, and immuno and gene therapy protocols. Can be used by both novice and experienced scientists and clinicians. Bartlett is affiliated with the Department of Surgery at the University of Glasgow, Scotland. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Read More
If there is one aspect of current cancer research that represents a major ch- lenge in both novice and experienced researchers, it is the rapid advance in our understanding of the disease. Researchers can be required to switch from analysis of gene expression to kinetics of protein activation, from genetic studies to the analysis of protein funtion. Cancers are highly complex disease systems and researchers aiming to understand the functioning of cancer systems require access to a wide range of laboratory techiques from a broad range of research disciplines. Increasingly, however, published methods are incomplete or refer back to a series of previous publications each containing only a small part of the complete pro- col. The aim of Ovarian Cancer: Methods and Protocols is to provide for ovarian cancer researchers in the first instance, a laboratory handbook that will facilitate research into cancer systems by providing a series of expert protocols, with proven efficacy, across a broad range of technical expertise. Thus, there are sections on tumor genetics and cellular signal transduction, as well as sections on apoptosis and RNA analysis. The value of Ovarian Cancer: Methods and Protocols to the ovarian cancer researcher will, I trust, be considerably enhanced by (1) the provision of a series of overviews relating to the biology, diagnosis, and treatment of this important neoplasm, and (2) the provision of a series of technical overviews introducing each part that provides an expert review of the applications and pitfalls of the various techniques included.
Read Less