Description
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
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Introduces a wide-ranging selection of the analytical and preparative techniques most frequently used by research workers in the field of molecular biology. Describes both the theory and the application of the techniques and includes references for those seeking detailed laboratory protocols. The techniques covered include extraction, separation, detection, and characterization of nucleic acids, gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling. The latter part of the book focuses on protein and related techniques. Annotation c. by Book News, Inc., Portland, Or.
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